| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRKAG3 (1-210) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC 1:50-1:200IF 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRKAG3 |
| Gene ID: |
53632 |
| Uniprot ID: |
AAKG3_HUMAN |
| Immunogen Region: |
1-210 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human PRKAG3 (NP_059127.2). |
| Immunogen Sequence: |
MEPGLEHALRRTPSWSSLGG SEHQEMSFLEQENSSSWPSP AVTSSSERIRGKRRAKALRW TRQKSVEEGEPPGQGEGPRS RPAAESTGLEATFPKTTPLA QADPAGVGTPPTGWDCLPSD CTASAAGSSTDDVELATEFP ATEAWECELEGLLEERPALC LSPQAPFPKLGWDDELRKPG AQIYMRFMQEHTCYDAMATS SKLVIFDTML |
| Tissue Specificity | Skeletal muscle, with weak expression in heart and pancreas. |
| Post Translational Modifications | Phosphorylated by ULK1.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism. It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Gamma-3Ampk Gamma3Ampk Subunit Gamma-3 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Gamma-3 antibodyAnti-Ampk Gamma3 antibodyAnti-Ampk Subunit Gamma-3 antibodyAnti-PRKAG3 antibodyAnti-AMPKG3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
