| Short Description : | Rabbit polyclonal PRG4 (1264-1363) antibody for use in WB and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRG4 |
| Gene ID: | 10216 |
| Uniprot ID: | PRG4_HUMAN |
| Immunogen Region: | 1264-1363 |
| Immunogen Sequence: | ERAIGPSQTHTIRIQYSPAR LAYQDKGVLHNEVKVSILWR GLPNVVTSAISLPNIRKPDG YDYYAFSKDQYYNIDVPSRT ARAITTRSGQTLSKVWYNCP |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2). |
| Tissue Specificity | Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed. |
| Post Translational Modifications | N-glycosylated. O-glycosylated.contains glycosaminoglycan chondroitin sulfate and keratan sulfate. O-glycosylated with sialylated oligosaccharides which are predominantly represented by the monosialylated core type I structure, NeuNAcalpha2-3Galbeta1-3GalNAc, with smaller amounts of disialylated O-glycans. The disulfide bond between Cys-1146 and Cys-1403 is essential for protein cleavage. Proteolytically cleaved by cathepsin CTSG. |
| Function | Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages. |
| Protein Name | Proteoglycan 4LubricinMegakaryocyte-Stimulating FactorSuperficial Zone Proteoglycan Cleaved Into - Proteoglycan 4 C-Terminal Part |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Proteoglycan 4 antibodyAnti-Lubricin antibodyAnti-Megakaryocyte-Stimulating Factor antibodyAnti-Superficial Zone Proteoglycan Cleaved Into - Proteoglycan 4 C-Terminal Part antibodyAnti-PRG4 antibodyAnti-MSF antibodyAnti-SZP antibody |
Information sourced from Uniprot.org
