• Immunofluorescence analysis of paraffin-embedded human breast cancer using PRG2 Rabbit polyclonal antibody (STJ11105497) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from THP-1 cells using PRG2 Rabbit polyclonal antibody (STJ11105497) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:45s.

Anti-PRG2 antibody (17-222) (STJ11105497)

SKU:
STJ11105497

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Bone Marrow Proteoglycan (17-222) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRG2
Gene ID: 5553
Uniprot ID: PRG2_HUMAN
Immunogen Region: 17-222
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-222 of human PRG2 (NP_002719.3).
Immunogen Sequence: LHLRSETSTFETPLGAKTLP EDEETPEQEMEETPCRELEE EEEWGSGSEDASKKDGAVES ISVPDMVDKNLTCPEEEDTV KVVGIPGCQTCRYLLVRSLQ TFSQAWFTCRRCYRGNLVSI HNFNINYRIQCSVSALNQGQ VWIGGRITGSGRCRRFQWVD GSRWNFAYWAAHQPWSRGGH CVALCTRGGHWRRAHCLRRL PFICSY
Tissue Specificity Detected in plasma and urine (at protein level). Detected in placenta (at protein level). High levels of the proform in placenta and pregnancy serum.in placenta, localized to X cells of septa and anchoring villi. Lower levels in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow and colon.
Post Translational Modifications Nitrated.
Function Cytotoxin and helminthotoxin. Also induces non-cytolytic histamine release from human basophils. Involved in antiparasitic defense mechanisms and immune hypersensitivity reactions. The proform acts as a proteinase inhibitor, reducing the activity of PAPPA.
Protein Name Bone Marrow Proteoglycan
Bmpg
Proteoglycan 2 Cleaved Into - Eosinophil Granule Major Basic Protein
Embp
Mbp
Pregnancy-Associated Major Basic Protein
Database Links Reactome: R-HSA-6798695
Cellular Localisation Bone Marrow Proteoglycan: Secreted
The Proform Is Secreted
Eosinophil Granule Major Basic Protein: Cytoplasmic Vesicle
Secretory Vesicle
The Mature Protein Is Found In The Matrix Of The Eosinophil's Large Specific Granule (Crystalloid Core)
Alternative Antibody Names Anti-Bone Marrow Proteoglycan antibody
Anti-Bmpg antibody
Anti-Proteoglycan 2 Cleaved Into - Eosinophil Granule Major Basic Protein antibody
Anti-Embp antibody
Anti-Mbp antibody
Anti-Pregnancy-Associated Major Basic Protein antibody
Anti-PRG2 antibody
Anti-MBP antibody

Information sourced from Uniprot.org

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