STJ73581 (0. 3µg/ml) staining of Human Breast cancer lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-prepro-transforming growth factor beta-2 antibody (Internal) (STJ73581)

SKU:
STJ73581-100
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Short Description :Goat polyclonal antibody anti-prepro-transforming growth factor beta-2 (Internal) is suitable for use in ELISA and Western Blot research applications.
Applications:Pep-ELISA/WB
Host:Goat
Reactivity:Human/Dog/Pig/Cow
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:0.5 mg/mL
Purification:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution Range:Peptide ELISA: antibody detection limit dilution 1:32000.
WB: Approx 55kDa band observed in Human Breast cancer lysates (calculated MW of 50.6kDa according to NP_001129071.1;). This molecular weight is routinely observed by other sources. Recomme
Formulation:0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage Instruction:Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol:TGFB2
Gene ID:7042
Uniprot ID:TGFB2_HUMAN
Accession Number:NP_001129071.1; NP_003229.1
Immunogen Region:Internal
Immunogen Sequence:STYTSGDQKTIKSTR
Specificity:This antibody is expected to recognize the part representing the latency-associated peptide.
Function Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
Protein Name Transforming Growth Factor Beta-2 Proprotein
Cetermin
Glioblastoma-Derived T-Cell Suppressor Factor
G-Tsf Cleaved Into - Latency-Associated Peptide
Lap - Transforming Growth Factor Beta-2
Tgf-Beta-2
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-2129379
Reactome: R-HSA-2173789
Reactome: R-HSA-3000178
Reactome: R-HSA-9839389
Cellular Localisation Latency-Associated Peptide: Secreted
Extracellular Space
Extracellular Matrix
Transforming Growth Factor Beta-2: Secreted
Alternative Antibody Names Anti-Transforming Growth Factor Beta-2 Proprotein antibody
Anti-Cetermin antibody
Anti-Glioblastoma-Derived T-Cell Suppressor Factor antibody
Anti-G-Tsf Cleaved Into - Latency-Associated Peptide antibody
Anti-Lap - Transforming Growth Factor Beta-2 antibody
Anti-Tgf-Beta-2 antibody
Anti-TGFB2 antibody

Information sourced from Uniprot.org

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