• STJ72074 (0. 1µg/ml) staining of A431 (A) , (2ug/ml) K562 (B) and (1ug/ml) Nuclear K562 (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ72074 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72074 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PRDM1/MEL1 antibody (Internal) (STJ72074)

SKU:
STJ72074-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PRDM1/MEL1 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei of HeLa cells. 10µg/ml
FC-Flow cytometric analysis of A431 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:1000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PRDM1
Gene ID: 639
Uniprot ID: PRDM1_HUMAN
Immunogen Region: Internal
Accession Number: NP_001189.2; NP_878911.1
Specificity: This antibody is expected to recognize both reported isoforms (NP_001189.2; NP_878911.1).
Immunogen Sequence: DISDNADRLEDVED
Function Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection. Binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions. Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1.
Protein Name Pr Domain Zinc Finger Protein 1
Blimp-1
Beta-Interferon Gene Positive Regulatory Domain I-Binding Factor
Pr Domain-Containing Protein 1
Positive Regulatory Domain I-Binding Factor 1
Prdi-Bf1
Prdi-Binding Factor 1
Database Links Reactome: R-HSA-6804754
Reactome: R-HSA-9701898
Reactome: R-HSA-9827857
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 1 antibody
Anti-Blimp-1 antibody
Anti-Beta-Interferon Gene Positive Regulatory Domain I-Binding Factor antibody
Anti-Pr Domain-Containing Protein 1 antibody
Anti-Positive Regulatory Domain I-Binding Factor 1 antibody
Anti-Prdi-Bf1 antibody
Anti-Prdi-Binding Factor 1 antibody
Anti-PRDM1 antibody
Anti-BLIMP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance