Anti-PRDM1 antibody (70-210) (STJ116165)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRDM1 (70-210) antibody for use in WB, IF, ICC and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRDM1 |
| Gene ID | 639 |
| Uniprot ID | PRDM1_HUMAN |
| Immunogen Region | 70-210 |
| Immunogen Sequence | GADGGTSVQAEASLPRNLLF KYATNSEEVIGVMSKEYIPK GTRFGPLIGEIYTNDTVPKN ANRKYFWRIYSRGELHHFID GFNEEKSNWMRYVNPAHSPR EQNLAACQNGMNIYFYTIKP IPANQELLVWYCRDFAERLH Y |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 70-210 of human PRDM1 (NP_001189.2). |
Additional Info
| Post Translational Modifications | Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation. Can be sumoylated with SUMO1 and SUMO2 by PML. Degradation of the wild-type protein mostly depends upon sumoylation, rather than ubiquitination. Desumoylated by SENP1 and SENP6. Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. |
| Function | Transcription factor that mediates a transcriptional program in various innate and adaptive immune tissue-resident lymphocyte T cell types such as tissue-resident memory T (Trm), natural killer (trNK) and natural killer T (NKT) cells and negatively regulates gene expression of proteins that promote the egress of tissue-resident T-cell populations from non-lymphoid organs. Plays a role in the development, retention and long-term establishment of adaptive and innate tissue-resident lymphocyte T cell types in non-lymphoid organs, such as the skin and gut, but also in other nonbarrier tissues like liver and kidney, and therefore may provide immediate immunological protection against reactivating infections or viral reinfection. Binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. Associates with the transcriptional repressor ZNF683 to chromatin at gene promoter regions. Binds to the promoter and acts as a transcriptional repressor of IRF8, thereby promotes transcription of osteoclast differentiation factors such as NFATC1 and EEIG1. |
| Protein Name | Pr Domain Zinc Finger Protein 1Blimp-1Beta-Interferon Gene Positive Regulatory Domain I-Binding FactorPr Domain-Containing Protein 1Positive Regulatory Domain I-Binding Factor 1Prdi-Bf1Prdi-Binding Factor 1 |
| Database Links | Reactome: R-HSA-6804754Reactome: R-HSA-9701898Reactome: R-HSA-9827857 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Pr Domain Zinc Finger Protein 1 antibodyAnti-Blimp-1 antibodyAnti-Beta-Interferon Gene Positive Regulatory Domain I-Binding Factor antibodyAnti-Pr Domain-Containing Protein 1 antibodyAnti-Positive Regulatory Domain I-Binding Factor 1 antibodyAnti-Prdi-Bf1 antibodyAnti-Prdi-Binding Factor 1 antibodyAnti-PRDM1 antibodyAnti-BLIMP1 antibody |
Information sourced from Uniprot.org