| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP4R1 |
| Gene ID: | 9989 |
| Uniprot ID: | PP4R1_HUMAN |
| Immunogen Region: | 324-647 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1). |
| Immunogen Sequence: | NPSSSGQYFKEESKSSEEMS VENKNRTRDQEAPEDVQVRP EDTPSDLSVSNSSVILENTM EDHAAEASGKPLGEISVPLD SSLLCTLSSESHQEAASNEN DKKPGNYKSMLRPEVGTTSQ DSALLDQELYNSFHFWRTPL PEIDLDIELEQNSGGKPSPE GPEEESEGPVPSSPNITMAT RKELEEMIENLEPHIDDPDV KAQVEVLSAALRASSLDAHE ETISIEKRSDLQDELDINE |
| Tissue Specificity | Widely expressed with high expression in cultured mesangial cells. Isoform 1 and isoform 2 are expressed in renal tissues. |
| Function | Regulatory subunit of serine/threonine-protein phosphatase 4. May play a role in regulation of cell division in renal glomeruli. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. Plays a role in the inhibition of TNF-induced NF-kappa-B activation by regulating the dephosphorylation of TRAF2. (Microbial infection) Participates in merkel polyomavirus-mediated inhibition of NF-kappa-B by bridging viral small tumor antigen with NEMO. |
| Protein Name | Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 1 antibodyAnti-PPP4R1 antibodyAnti-MEG1 antibodyAnti-PP4R1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89743/358218/STJ11100807_1__82919.1713122544.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and TXNDC5 knockout (KO) 293T cells, using [KO Validated] TXNDC5 Rabbit polyclonal antibody (STJ11100807) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
