• Immunofluorescence analysis of U-2 OS cells using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PP2A-B56 Delta/PR61 Delta/PPP2R5D in paraffin-embedded mouse kidney using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PP2A-B56 Delta/PR61 Delta/PPP2R5D in paraffin-embedded human thyroid cancer using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PP2A-B56 Delta/PR61 Delta/PPP2R5D in paraffin-embedded rat brain using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PP2A-B56 Delta/PR61 Delta/PPP2R5D Rabbit polyclonal antibody (STJ118153) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PPP2R5D antibody (450-550) (STJ118153)

SKU:
STJ118153

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP2R5D (450-550) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2R5D
Gene ID: 5528
Uniprot ID: 2A5D_HUMAN
Immunogen Region: 450-550
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 450-550 of human PP2A-B56 Delta/PR61 Delta/PP2A-B56 Delta/PR61 Delta/PPP2R5D (NP_006236.1).
Immunogen Sequence: LYRNSKSHWNKTIHGLIYNA LKLFMEMNQKLFDDCTQQYK AEKQKGRFRMKEREEMWQKI EELARLNPQYPMFRAPPPLP PVYSMETETPTAEDIQLLKR T
Tissue Specificity Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Protein Name Serine/Threonine-Protein Phosphatase 2a 56 Kda Regulatory Subunit Delta Isoform
Pp2a B Subunit Isoform B'-Delta
Pp2a B Subunit Isoform B56-Delta
Pp2a B Subunit Isoform Pr61-Delta
Pp2a B Subunit Isoform R5-Delta
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-163685
Reactome: R-HSA-163767
Reactome: R-HSA-180024
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-389513
Reactome: R-HSA-432142
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5663220
Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6811558
Reactome: R-HSA-68877
Reactome: R-HSA-9634600
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Nuclear In Interphase
Nuclear During Mitosis
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a 56 Kda Regulatory Subunit Delta Isoform antibody
Anti-Pp2a B Subunit Isoform B'-Delta antibody
Anti-Pp2a B Subunit Isoform B56-Delta antibody
Anti-Pp2a B Subunit Isoform Pr61-Delta antibody
Anti-Pp2a B Subunit Isoform R5-Delta antibody
Anti-PPP2R5D antibody

Information sourced from Uniprot.org

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