| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:10-1:100 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP2R1A |
| Gene ID: | 5518 |
| Uniprot ID: | 2AAA_HUMAN |
| Immunogen Region: | 1-270 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PP2A-Aalpha/PR65 Alpha/PP2A-A Alpha/PR65 Alpha/PPP2R1A (NP_055040.2). |
| Immunogen Sequence: | MAAADGDDSLYPIAVLIDEL RNEDVQLRLNSIKKLSTIAL ALGVERTRSELLPFLTDTIY DEDEVLLALAEQLGTFTTLV GGPEYVHCLLPPLESLATVE ETVVRDKAVESLRAISHEHS PSDLEAHFVPLVKRLAGGDW FTSRTSACGLFSVCYPRVSS AVKAELRQYFRNLCSDDTPM VRRAAASKLGEFAKVLELDN VKSEIIPMFSNLASDEQDSV RLLAVEACVNIAQLLPQED |
| Function | The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis. Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway. Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation. Regulates the recruitment of the SKA complex to kinetochores. |
| Protein Name | Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Alpha Isoform Medium Tumor Antigen-Associated 61 Kda Protein Pp2a Subunit A Isoform Pr65-Alpha Pp2a Subunit A Isoform R1-Alpha |
| Database Links | Reactome: R-HSA-113501 Reactome: R-HSA-1295596 Reactome: R-HSA-141444 Reactome: R-HSA-163685 Reactome: R-HSA-163767 Reactome: R-HSA-180024 Reactome: R-HSA-195253 Reactome: R-HSA-196299 Reactome: R-HSA-198753 Reactome: R-HSA-202670 Reactome: R-HSA-2465910 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-2565942 Reactome: R-HSA-2995383 Reactome: R-HSA-380259 Reactome: R-HSA-380270 Reactome: R-HSA-380284 Reactome: R-HSA-380320 Reactome: R-HSA-389513 Reactome: R-HSA-432142 Reactome: R-HSA-4641262 Reactome: R-HSA-5339716 Reactome: R-HSA-5358747 Reactome: R-HSA-5358749 Reactome: R-HSA-5358751 Reactome: R-HSA-5358752 Reactome: R-HSA-5467337 Reactome: R-HSA-5467340 Reactome: R-HSA-5467348 Reactome: R-HSA-5620912 Reactome: R-HSA-5663220 Reactome: R-HSA-5673000 Reactome: R-HSA-5675221 Reactome: R-HSA-6804757 Reactome: R-HSA-6811558 Reactome: R-HSA-68877 Reactome: R-HSA-69231 Reactome: R-HSA-69273 Reactome: R-HSA-8854518 Reactome: R-HSA-9634600 Reactome: R-HSA-9648025 Reactome: R-HSA-975957 Reactome: R-HSA-9833482 |
| Cellular Localisation | Cytoplasm Nucleus Chromosome Centromere Lateral Cell Membrane Cell Projection Dendrite Centromeric Localization Requires The Presence Of Bub1 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Alpha Isoform antibody Anti-Medium Tumor Antigen-Associated 61 Kda Protein antibody Anti-Pp2a Subunit A Isoform Pr65-Alpha antibody Anti-Pp2a Subunit A Isoform R1-Alpha antibody Anti-PPP2R1A antibody |
Information sourced from Uniprot.org