| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:10-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP2R1A |
| Gene ID: | 5518 |
| Uniprot ID: | 2AAA_HUMAN |
| Immunogen Region: | 1-270 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human PP2A-Aalpha/PR65 Alpha/PP2A-A Alpha/PR65 Alpha/PPP2R1A (NP_055040.2). |
| Immunogen Sequence: | MAAADGDDSLYPIAVLIDEL RNEDVQLRLNSIKKLSTIAL ALGVERTRSELLPFLTDTIY DEDEVLLALAEQLGTFTTLV GGPEYVHCLLPPLESLATVE ETVVRDKAVESLRAISHEHS PSDLEAHFVPLVKRLAGGDW FTSRTSACGLFSVCYPRVSS AVKAELRQYFRNLCSDDTPM VRRAAASKLGEFAKVLELDN VKSEIIPMFSNLASDEQDSV RLLAVEACVNIAQLLPQED |
| Function | The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis. Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway. Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation. Regulates the recruitment of the SKA complex to kinetochores. |
| Protein Name | Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Alpha IsoformMedium Tumor Antigen-Associated 61 Kda ProteinPp2a Subunit A Isoform Pr65-AlphaPp2a Subunit A Isoform R1-Alpha |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-1295596Reactome: R-HSA-141444Reactome: R-HSA-163685Reactome: R-HSA-163767Reactome: R-HSA-180024Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2465910Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-2995383Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-389513Reactome: R-HSA-432142Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5620912Reactome: R-HSA-5663220Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6804757Reactome: R-HSA-6811558Reactome: R-HSA-68877Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-8854518Reactome: R-HSA-9634600Reactome: R-HSA-9648025Reactome: R-HSA-975957Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereLateral Cell MembraneCell ProjectionDendriteCentromeric Localization Requires The Presence Of Bub1 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Alpha Isoform antibodyAnti-Medium Tumor Antigen-Associated 61 Kda Protein antibodyAnti-Pp2a Subunit A Isoform Pr65-Alpha antibodyAnti-Pp2a Subunit A Isoform R1-Alpha antibodyAnti-PPP2R1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
