• Immunofluorescence analysis of U-2 OS cells using PP2A alpha + beta Rabbit monoclonal antibody (STJ11102318) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PP2A alpha + beta Rabbit monoclonal antibody (STJ11102318) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PP2A alpha + beta Rabbit monoclonal antibody (STJ11102318) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using PP2A alpha + beta Rabbit monoclonal antibody (STJ11102318) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PP2A alpha + beta Rabbit monoclonal antibody (STJ11102318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PPP2CA/PPP2CB antibody (200-309) [S8MR] (STJ11102318)

SKU:
STJ11102318

£129.50 - £574.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2CA
PPP2CB
Gene ID: 5515
5516
Uniprot ID: PP2AA_HUMAN
PP2AB_HUMAN
Immunogen Region: 200-309
Specificity: A synthetic peptide corresponding to a sequence within amino acids 200-309 of human PP2A alpha + beta (P62714).
Immunogen Sequence: WSDPDDRGGWGISPRGAGYT FGQDISETFNHANGLTLVSR AHQLVMEGYNWCHDRNVVTI FSAPNYCYRCGNQAAIMELD DTLKYSFLQFDPAPRRGEPH VTRRTPDYFL
Tissue Specificity
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibody
Anti-Pp2a-Alpha antibody
Anti-Replication Protein C antibody
Anti-Rp-C antibody
Anti-PPP2CA antibody
Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Beta Isoform antibody
Anti-Pp2a-Beta antibody
Anti-PPP2CB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance