| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Nuclear inhibitor of protein phosphatase 1 (196-245) is suitable for use in Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP1R8 |
| Gene ID: | 5511 |
| Uniprot ID: | PP1R8_HUMAN |
| Immunogen Region: | 196-245 |
| Specificity: | NIPP1 Polyclonal Antibody detects endogenous levels of NIPP1 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human PPP1R8. AA range:196-245 |
| Tissue Specificity | Ubiquitously expressed, with highest levels in heart and skeletal muscle, followed by brain, placenta, lung, liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (>90%) and isoform Beta were found in brain, heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes, and has been found in 293 embryonic kidney cells. |
| Post Translational Modifications | May be inactivated by phosphorylation on Ser-199 or Ser-204. Phosphorylated by Lyn in vitro on Tyr-264, and also on Tyr-335 in the presence of RNA. |
| Function | Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. |
| Protein Name | Nuclear Inhibitor Of Protein Phosphatase 1 Nipp-1 Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay Ard-1 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus Nucleus Speckle Primarily But Not Exclusively Nuclear Isoform Gamma: Cytoplasm Found Mainly In The Cytoplasm |
| Alternative Antibody Names | Anti-Nuclear Inhibitor Of Protein Phosphatase 1 antibody Anti-Nipp-1 antibody Anti-Protein Phosphatase 1 Regulatory Inhibitor Subunit 8 Includes - Activator Of Rna Decay antibody Anti-Ard-1 antibody Anti-PPP1R8 antibody Anti-ARD1 antibody Anti-NIPP1 antibody |
Information sourced from Uniprot.org