• Western blot analysis of various lysates using PPP1R14B Rabbit polyclonal antibody (STJ116879) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PPP1R14B antibody (68-147) (STJ116879)

SKU:
STJ116879

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP1R14B (68-147) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R14B
Gene ID: 26472
Uniprot ID: PP14B_HUMAN
Immunogen Region: 68-147
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 68-147 of human PPP1R14B (NP_619634.1).
Immunogen Sequence: RLNLEEWILEQLTRLYDCQE EEIPELEIDVDELLDMESDD ARAARVKELLVDCYKPTEAF ISGLLDKIRGMQKLSTPQKK
Tissue Specificity Ubiquitous. Expressed at low levels.
Post Translational Modifications Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown Ser is also phosphorylated by PKC (in vitro).
Function Inhibitor of PPP1CA. Has over 50-fold higher inhibitory activity when phosphorylated.
Protein Name Protein Phosphatase 1 Regulatory Subunit 14b
Phospholipase C-Beta-3 Neighbouring Gene Protein
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 14b antibody
Anti-Phospholipase C-Beta-3 Neighbouring Gene Protein antibody
Anti-PPP1R14B antibody
Anti-PLCB3N antibody
Anti-PNG antibody

Information sourced from Uniprot.org

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