| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PPP1R12A (710-970) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPP1R12A |
| Gene ID: |
4659 |
| Uniprot ID: |
MYPT1_HUMAN |
| Immunogen Region: |
710-970 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 710-970 of human PPP1R12A (NP_002471.1). |
| Immunogen Sequence: |
TIGRSRSTRTREQENEEKEK EEKEKQDKEKQEEKKESETS REDEYKQKYSRTYDETYQRY RPVSTSSSTTPSSSLSTMSS SLYASSQLNRPNSLVGITSA YSRGITKENEREGEKREEEK EGEDKSQPKSIRERRRPREK RRSTGVSFWTQDSDENEQEQ QSDTEEGSNKKETQTDSISR YETSSTSAGDRYDSLLGRSG SYSYLEERKPYSSRLEKDDS TDFKKLYEQILAENEKLKA |
| Tissue Specificity | Expressed in striated muscles, specifically in type 2a fibers (at protein level). |
| Post Translational Modifications | Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. |
| Function | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 12aMyosin Phosphatase-Targeting Subunit 1Myosin Phosphatase Target Subunit 1Protein Phosphatase Myosin-Binding Subunit |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberAlso Along Actomyosin Filaments |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibodyAnti-Myosin Phosphatase-Targeting Subunit 1 antibodyAnti-Myosin Phosphatase Target Subunit 1 antibodyAnti-Protein Phosphatase Myosin-Binding Subunit antibodyAnti-PPP1R12A antibodyAnti-MBS antibodyAnti-MYPT1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
