Anti-PPP1R12A antibody (710-970) (STJ28783)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PPP1R12A (710-970) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP1R12A |
| Gene ID | 4659 |
| Uniprot ID | MYPT1_HUMAN |
| Immunogen Region | 710-970 |
| Immunogen Sequence | TIGRSRSTRTREQENEEKEK EEKEKQDKEKQEEKKESETS REDEYKQKYSRTYDETYQRY RPVSTSSSTTPSSSLSTMSS SLYASSQLNRPNSLVGITSA YSRGITKENEREGEKREEEK EGEDKSQPKSIRERRRPREK RRSTGVSFWTQDSDENEQEQ QSDTEEGSNKKETQTDSISR YETSSTSAGDRYDSLLGRSG SYSYLEERKPYSSRLEKDDS TDFKKLYEQILAENEKLKA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 710-970 of human PPP1R12A (NP_002471.1). |
Additional Info
| Tissue Specificity | Expressed in striated muscles, specifically in type 2a fibers (at protein level). |
| Post Translational Modifications | Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A. |
| Function | Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 12aMyosin Phosphatase-Targeting Subunit 1Myosin Phosphatase Target Subunit 1Protein Phosphatase Myosin-Binding Subunit |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-5625740Reactome: R-HSA-5625900Reactome: R-HSA-5627117Reactome: R-HSA-5627123 |
| Cellular Localisation | CytoplasmCytoskeletonStress FiberAlso Along Actomyosin Filaments |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibodyAnti-Myosin Phosphatase-Targeting Subunit 1 antibodyAnti-Myosin Phosphatase Target Subunit 1 antibodyAnti-Protein Phosphatase Myosin-Binding Subunit antibodyAnti-PPP1R12A antibodyAnti-MBS antibodyAnti-MYPT1 antibody |
Information sourced from Uniprot.org