Western blot analysis of various lysates using (STJ11105751) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-PPM1E antibody (500-600) [S5751RM] (STJ11105751)

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STJ11105751
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5751RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPM1E
Gene ID: 22843
Uniprot ID: PPM1E_HUMAN
Immunogen Region: 500-600
Specificity: A synthetic peptide corresponding to a sequence within amino acids 500-600 of human PPM1E (Q8WY54).
Immunogen Sequence: ESDWTENSFQGGQEDGGDDK ENHGECKRPWPQHQCSAPAD LGYDGRVDSFTDRTSLSPGS QINVLEDPGYLDLTQIEASK PHSAQFLLPVEMFGPGAPKK A
Function Protein phosphatase that inactivates multifunctional CaM kinases such as CAMK4 and CAMK2. Dephosphorylates and inactivates PAK. May play a role in the inhibition of actin fiber stress breakdown and in morphological changes driven by TNK2/CDC42. Dephosphorylates PRKAA2.
Protein Name Protein Phosphatase 1e
Ca(2+/Calmodulin-Dependent Protein Kinase Phosphatase N
Camkp-N
Camkp-Nucleus
Camkn
Partner Of Pix 1
Partner Of Pix-Alpha
Partner Of Pixa
Database Links Reactome: R-HSA-9617324
Cellular Localisation Nucleus
Cytoplasm
A Truncated Form
Major Form
With The C-Terminal Part Missing
Is Mostly Found In The Cytoplasm And A Little In The Nucleus
The Full-Length
Minor Form
Is Found In The Nucleus
Alternative Antibody Names Anti-Protein Phosphatase 1e antibody
Anti-Ca(2+/Calmodulin-Dependent Protein Kinase Phosphatase N antibody
Anti-Camkp-N antibody
Anti-Camkp-Nucleus antibody
Anti-Camkn antibody
Anti-Partner Of Pix 1 antibody
Anti-Partner Of Pix-Alpha antibody
Anti-Partner Of Pixa antibody
Anti-PPM1E antibody
Anti-CAMKN antibody
Anti-KIAA1072 antibody
Anti-POPX1 antibody

Information sourced from Uniprot.org

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