Anti-PPIF antibody (30-207) (STJ11105648)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Peptidyl-Prolyl Cis-Trans Isomerase F-Mitochondrial (30-207) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPIF |
| Gene ID | 10105 |
| Uniprot ID | PPIF_HUMAN |
| Immunogen Region | 30-207 |
| Immunogen Sequence | CSKGSGDPSSSSSSGNPLVY LDVDANGKPLGRVVLELKAD VVPKTAENFRALCTGEKGFG YKGSTFHRVIPSFMCQAGDF TNHNGTGGKSIYGSRFPDEN FTLKHVGPGVLSMANAGPNT NGSQFFICTIKTDWLDGKHV VFGHVKEGMDVVKKIESFGS KSGRTSKKIVITDCGQLS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human PPIF (NP_005720.1). |
Additional Info
| Post Translational Modifications | Acetylated at Lys-167.deacetylated at Lys-167 by SIRT3. |
| Function | PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis.the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase F - MitochondrialPpiase FCyclophilin DCyp-DCypdCyclophilin FMitochondrial CyclophilinCyp-MRotamase F |
| Database Links | |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Peptidyl-Prolyl Cis-Trans Isomerase F - Mitochondrial antibodyAnti-Ppiase F antibodyAnti-Cyclophilin D antibodyAnti-Cyp-D antibodyAnti-Cypd antibodyAnti-Cyclophilin F antibodyAnti-Mitochondrial Cyclophilin antibodyAnti-Cyp-M antibodyAnti-Rotamase F antibodyAnti-PPIF antibodyAnti-CYP3 antibody |
Information sourced from Uniprot.org