• Immunofluorescence analysis of U2OS cells using PPAR Gamma Rabbit polyclonal antibody (STJ25069) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PPAR Gamma in paraffin-embedded mouse liver using PPAR Gamma Rabbit polyclonal antibody (STJ25069) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PPAR Gamma in paraffin-embedded mouse heart using PPAR Gamma Rabbit polyclonal antibody (STJ25069) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PPARG antibody (115-132) (STJ25069)

SKU:
STJ25069

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Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPAR gamma (115-132) is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPARG
Gene ID: 5468
Uniprot ID: PPARG_HUMAN
Immunogen Region: 115-132
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 115-132 of human PPAR Gamma (NP_056953.2).
Immunogen Sequence: YYSEKTQLYNKPHE
Tissue Specificity Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
Post Translational Modifications O-GlcNAcylation at Thr-84 reduces transcriptional activity in adipocytes. Phosphorylated in basal conditions and dephosphorylated when treated with the ligand. May be dephosphorylated by PPP5C. The phosphorylated form may be inactive and dephosphorylation at Ser-112 induces adipogenic activity. Ubiquitinated by E3 ubiquitin-protein ligase complex containing FBXO9.leading to proteasomal degradation.
Function Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of BMAL1 in the blood vessels. (Microbial infection) Upon treatment with M.tuberculosis or its lipoprotein LpqH, phosphorylation of MAPK p38 and IL-6 production are modulated, probably via this protein.
Protein Name Peroxisome Proliferator-Activated Receptor Gamma
Ppar-Gamma
Nuclear Receptor Subfamily 1 Group C Member 3
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-8943724
Reactome: R-HSA-9022707
Cellular Localisation Nucleus
Cytoplasm
Redistributed From The Nucleus To The Cytosol Through A Map2k1/Mek1-Dependent Manner
Noct Enhances Its Nuclear Translocation
Alternative Antibody Names Anti-Peroxisome Proliferator-Activated Receptor Gamma antibody
Anti-Ppar-Gamma antibody
Anti-Nuclear Receptor Subfamily 1 Group C Member 3 antibody
Anti-PPARG antibody
Anti-NR1C3 antibody

Information sourced from Uniprot.org

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