• Immunofluorescence analysis of HeLa cells, using PPAR-alpha Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from NIH/3T3 cells, using PPAR-alpha Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1, PPAR-Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Western blot analysis of 3T3 cells using PPAR-Alpha Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, PPAR-Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, PPAR-Alpha Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of mouse-kidney tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of mouse-kidney tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-spleen tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of rat-lung tissue. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Hela cell. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PPARA antibody (6-55 aa) (STJ95200)

SKU:
STJ95200

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Peroxisome proliferator-activated receptor alpha (6-55 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:10000-20000
IHC 1:50-300
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPARA
Gene ID: 5465
Uniprot ID: PPARA_HUMAN
Immunogen Region: 6-55 aa
Specificity: PPAR-Alpha Polyclonal Antibody detects endogenous levels of PPAR-Alpha protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PPAR-alpha at the amino acid range 6-55
Function Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Protein Name Peroxisome Proliferator-Activated Receptor Alpha
Ppar-Alpha
Nuclear Receptor Subfamily 1 Group C Member 1
Database Links Reactome: R-HSA-1368082
Reactome: R-HSA-1368108
Reactome: R-HSA-1989781
Reactome: R-HSA-2151201
Reactome: R-HSA-2426168
Reactome: R-HSA-381340
Reactome: R-HSA-383280
Reactome: R-HSA-400206
Reactome: R-HSA-400253
Reactome: R-HSA-4090294
Reactome: R-HSA-9707564
Reactome: R-HSA-9707616
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Peroxisome Proliferator-Activated Receptor Alpha antibody
Anti-Ppar-Alpha antibody
Anti-Nuclear Receptor Subfamily 1 Group C Member 1 antibody
Anti-PPARA antibody
Anti-NR1C1 antibody
Anti-PPAR antibody

Information sourced from Uniprot.org

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