• Western blot analysis of lysates from HeLa cells, using OCT3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of HeLa cells using Oct-3/4 Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using OCT3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using Oct-3/4 Polyclonal Antibody diluted at 1：1000

Anti-POU5F1 antibody (191-240) (STJ94598)

SKU:
STJ94598

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU domain, class 5, transcription factor 1 (191-240) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU5F1
Gene ID: 5460
Uniprot ID: PO5F1_HUMAN
Immunogen Region: 191-240
Specificity: Oct-3/4 Polyclonal Antibody detects endogenous levels of Oct-3/4 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human OCT3. AA range:191-240
Tissue Specificity Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Post Translational Modifications Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Protein Name Pou Domain - Class 5 - Transcription Factor 1
Octamer-Binding Protein 3
Oct-3
Octamer-Binding Protein 4
Oct-4
Octamer-Binding Transcription Factor 3
Otf-3
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-9754189
Reactome: R-HSA-9827857
Cellular Localisation Cytoplasm
Nucleus
Expressed In A Diffuse And Slightly Punctuate Pattern
Colocalizes With Mapk8 And Mapk9 In The Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 5 - Transcription Factor 1 antibody
Anti-Octamer-Binding Protein 3 antibody
Anti-Oct-3 antibody
Anti-Octamer-Binding Protein 4 antibody
Anti-Oct-4 antibody
Anti-Octamer-Binding Transcription Factor 3 antibody
Anti-Otf-3 antibody
Anti-POU5F1 antibody
Anti-OCT3 antibody
Anti-OCT4 antibody
Anti-OTF3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance