Anti-POU4F1 antibody (311-360 aa) (STJ91894)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human POU4F1 at the amino acid range 311-360
General Information
| Short Description | Rabbit polyclonal anti-POU domain, class 4, transcription factor 1 (311-360 aa) for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POU4F1 |
| Gene ID | 5457 |
| Uniprot ID | PO4F1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human POU4F1 at the amino acid range 311-360 |
| Immunogen Region | 311-360 aa |
| Specificity | Brn-3a Polyclonal Antibody detects endogenous levels of Brn-3a protein. |
Additional Info
| Function | Multifunctional transcription factor with different regions mediating its different effects. Acts by binding (via its C-terminal domain) to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of its target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that activation of some of these genes requires its N-terminal domain, maybe through a neuronal-specific cofactor. Activates BCL2 expression and protects neuronal cells from apoptosis (via the N-terminal domain). Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. Stimulates the binding affinity of the nuclear estrogene receptor ESR1 to DNA estrogen response element (ERE), and hence modulates ESR1-induced transcriptional activity. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Plays a role in TNFSF11-mediated terminal osteoclast differentiation. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site. Isoform 2: Able to act as transcription factor, cannot regulate the expression of the same subset of genes than isoform 1. Does not have antiapoptotic effect on neuronal cells. |
| Protein Name | Pou Domain - Class 4 - Transcription Factor 1Brain-Specific Homeobox/Pou Domain Protein 3aBrain-3aBrn-3aHomeobox/Pou Domain Protein Rdc-1Oct-T1 |
| Database Links | Reactome: R-HSA-6804759 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Pou Domain - Class 4 - Transcription Factor 1 antibodyAnti-Brain-Specific Homeobox/Pou Domain Protein 3a antibodyAnti-Brain-3a antibodyAnti-Brn-3a antibodyAnti-Homeobox/Pou Domain Protein Rdc-1 antibodyAnti-Oct-T1 antibodyAnti-POU4F1 antibodyAnti-BRN3A antibodyAnti-RDC1 antibody |
Information sourced from Uniprot.org