• Western blot analysis of various lysates using POU3F1 Rabbit polyclonal antibody (STJ11100601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POU3F1 antibody (1-260) (STJ11100601)

SKU:
STJ11100601

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU3F1 (1-260) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU3F1
Gene ID: 5453
Uniprot ID: PO3F1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human POU3F1 (NP_002690.3).
Immunogen Sequence: MATTAQYLPRGPGGGAGGTG PLMHPDAAAAAAAAAAAERL HAGAAYREVQKLMHHEWLGA GAGHPVGLAHPQWLPTGGGG GGDWAGGPHLEHGKAGGGGT GRADDGGGGGGFHARLVHQG AAHAGAAWAQGSTAHHLGPA MSPSPGASGGHQPQPLGLYA QAAYPGGGGGGLAGMLAAGG GGAGPGLHHALHEDGHEAQL EPSPPPHLGAHGHAHGHAHA GGLHAAAAHLHPGAGGGGS
Tissue Specificity Expressed in embryonal stem cells and in the developing brain.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. Acts as a transcriptional repressor of myelin-specific genes.
Protein Name Pou Domain - Class 3 - Transcription Factor 1
Octamer-Binding Protein 6
Oct-6
Octamer-Binding Transcription Factor 6
Otf-6
Pou Domain Transcription Factor Scip
Database Links Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 3 - Transcription Factor 1 antibody
Anti-Octamer-Binding Protein 6 antibody
Anti-Oct-6 antibody
Anti-Octamer-Binding Transcription Factor 6 antibody
Anti-Otf-6 antibody
Anti-Pou Domain Transcription Factor Scip antibody
Anti-POU3F1 antibody
Anti-OCT6 antibody
Anti-OTF6 antibody

Information sourced from Uniprot.org

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