• Western blot analysis of Daudi, using BOB1 Rabbit polyclonal antibody (STJ11105327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POU2AF1 antibody (150-250) (STJ11105327)

SKU:
STJ11105327

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pou Domain Class 2-Associating Factor 1 (150-250) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2AF1
Gene ID: 5450
Uniprot ID: OBF1_HUMAN
Immunogen Region: 150-250
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-250 of human BOB1 (NP_006226.2).
Immunogen Sequence: NVTTRSSATPAVGPPLEGPE HQAPLTYFPWPQPLSTLPTS TLQYQPPAPALPGPQFVQLP ISIPEPVLQDMEDPRRAASS LTIDKLLLEEEDSDAYALNH T
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
Protein Name Pou Domain Class 2-Associating Factor 1
B-Cell-Specific Coactivator Obf-1
Bob-1
Oca-B
Oct-Binding Factor 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain Class 2-Associating Factor 1 antibody
Anti-B-Cell-Specific Coactivator Obf-1 antibody
Anti-Bob-1 antibody
Anti-Oca-B antibody
Anti-Oct-Binding Factor 1 antibody
Anti-POU2AF1 antibody
Anti-BOB1 antibody
Anti-OBF1 antibody

Information sourced from Uniprot.org

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