• Immunohistochemistry analysis of paraffin-embedded mouse liver using POP4 antibody (STJ26679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using POP4 antibody (STJ26679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using POP4 antibody (STJ26679) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using POP4 antibody (STJ26679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POP4 antibody (1-220) (STJ26679)

SKU:
STJ26679

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POP4 (1-220) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POP4
Gene ID: 10775
Uniprot ID: RPP29_HUMAN
Immunogen Region: 1-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human POP4 (NP_006618.1).
Immunogen Sequence: MKSVIYHALSQKEANDSDVQ PSGAQRAEAFVRAFLKRSTP RMSPQAREDQLQRKAVVLEY FTRHKRKEKKKKAKGLSARQ RRELRLFDIKPEQQRYSLFL PLHELWKQYIRDLCSGLKPD TQPQMIQAKLLKADLHGAII SVTKSKCPSYVGITGILLQE TKHIFKIITKEDRLKVIPKL NCVFTVETDGFISYIYGSKF QLRSSERSAKKFKAKGTIDL
Function Component of ribonuclease P, a ribonucleoprotein complex that generates mature tRNA molecules by cleaving their 5'-ends.
Protein Name Ribonuclease P Protein Subunit P29
Hpop4
Database Links Reactome: R-HSA-6784531
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Ribonuclease P Protein Subunit P29 antibody
Anti-Hpop4 antibody
Anti-POP4 antibody
Anti-RPP29 antibody

Information sourced from Uniprot.org

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