Anti-POLRMT antibody (42-230) (STJ118054)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal POLRMT (42-230) antibody for use in WB, IF, ICC, IP and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLRMT |
| Gene ID | 5442 |
| Uniprot ID | RPOM_HUMAN |
| Immunogen Region | 42-230 |
| Immunogen Sequence | SSASPQEQDQDRRKDWGHVE LLEVLQARVRQLQAESVSEV VVNRVDVARLPECGSGDGSL QPPRKVQMGAKDATPVPCGR WAKILEKDKRTQQMRMQRLK AKLQMPFQSGEFKALTRRLQ VEPRLLSKQMAGCLEDCTRQ APESPWEEQLARLLQEAPGK LSLDVEQAPSGQHSQAQLSG QQQRLLAFF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3). |
Additional Info
| Function | DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Has DNA primase activity. Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL). |
| Protein Name | Dna-Directed Rna Polymerase - MitochondrialMtrpol |
| Database Links | Reactome: R-HSA-163282Reactome: R-HSA-2151201Reactome: R-HSA-9913635 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase - Mitochondrial antibodyAnti-Mtrpol antibodyAnti-POLRMT antibody |
Information sourced from Uniprot.org