• Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells, using 3 Mu g POLRMT antibody (STJ118054). Western blot was performed from the immunoprecipitate using POLRMT antibody (STJ118054) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using POLRMT Rabbit polyclonal antibody (STJ118054) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using POLRMT Rabbit polyclonal antibody (STJ118054) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using POLRMT Rabbit polyclonal antibody (STJ118054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-POLRMT antibody (42-230) (STJ118054)

SKU:
STJ118054

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POLRMT (42-230) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLRMT
Gene ID: 5442
Uniprot ID: RPOM_HUMAN
Immunogen Region: 42-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3).
Immunogen Sequence: SSASPQEQDQDRRKDWGHVE LLEVLQARVRQLQAESVSEV VVNRVDVARLPECGSGDGSL QPPRKVQMGAKDATPVPCGR WAKILEKDKRTQQMRMQRLK AKLQMPFQSGEFKALTRRLQ VEPRLLSKQMAGCLEDCTRQ APESPWEEQLARLLQEAPGK LSLDVEQAPSGQHSQAQLSG QQQRLLAFF
Function DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Has DNA primase activity. Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL).
Protein Name Dna-Directed Rna Polymerase - Mitochondrial
Mtrpol
Database Links Reactome: R-HSA-163282
Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase - Mitochondrial antibody
Anti-Mtrpol antibody
Anti-POLRMT antibody

Information sourced from Uniprot.org

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