| Host: | Rabbit |
| Applications: | WB/IF/ICC/IP/ELISA |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLRMT |
| Gene ID: | 5442 |
| Uniprot ID: | RPOM_HUMAN |
| Immunogen Region: | 42-230 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 42-230 of human POLRMT (NP_005026.3). |
| Immunogen Sequence: | SSASPQEQDQDRRKDWGHVE LLEVLQARVRQLQAESVSEV VVNRVDVARLPECGSGDGSL QPPRKVQMGAKDATPVPCGR WAKILEKDKRTQQMRMQRLK AKLQMPFQSGEFKALTRRLQ VEPRLLSKQMAGCLEDCTRQ APESPWEEQLARLLQEAPGK LSLDVEQAPSGQHSQAQLSG QQQRLLAFF |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of mitochondrial DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Has DNA primase activity. Catalyzes the synthesis of short RNA primers that are necessary for the initiation of lagging-strand DNA synthesis from the origin of light-strand DNA replication (OriL). |
| Protein Name | Dna-Directed Rna Polymerase - MitochondrialMtrpol |
| Database Links | Reactome: R-HSA-163282Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase - Mitochondrial antibodyAnti-Mtrpol antibodyAnti-POLRMT antibody |
Information sourced from Uniprot.org