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Anti-POLR2J antibody (1-117) (STJ25052)

Immunofluorescence analysis of A549 cells using POLR2J antibody (STJ25052).
Immunofluorescence analysis of U2OS cells using POLR2J antibody (STJ25052). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using POLR2J antibody (STJ25052) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-POLR2J antibody (1-117) (STJ25052)

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STJ25052

£112.50 - £524.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IF/ICC/ELISA
Reactivity:	Human/Mouse
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification:	Affinity purification
Concentration:	Lot specific
Dilution Range:	WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	POLR2J
Gene ID:	5439
Uniprot ID:	RPB11_HUMAN
Immunogen Region:	1-117
Specificity:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human POLR2J (NP_006225.1).
Immunogen Sequence:	MNAPPAFESFLLFEGEKKIT INKDTKVPNACLFTINKEDH TLGNIIKSQLLKDPQVLFAG YKVPHPLEHKIIIRVQTTPD YSPQEAFTNAITDLISELSL LEERFRVAIKDKQEGIE

Tissue Specificity	Ubiquitously expressed. High expression was found in heart and skeletal muscle.
Function	Core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates.
Protein Name	Dna-Directed Rna Polymerase Ii Subunit Rpb11-A Rna Polymerase Ii Subunit B11-A Rpb11a Dna-Directed Rna Polymerase Ii Subunit J-1 Rna Polymerase Ii 13.3 Kda Subunit
Database Links	Reactome: R-HSA-112382 Reactome: R-HSA-113418 Reactome: R-HSA-167152 Reactome: R-HSA-167158 Reactome: R-HSA-167160 Reactome: R-HSA-167161 Reactome: R-HSA-167162 Reactome: R-HSA-167172 Reactome: R-HSA-167200 Reactome: R-HSA-167238 Reactome: R-HSA-167242 Reactome: R-HSA-167243 Reactome: R-HSA-167246 Reactome: R-HSA-167287 Reactome: R-HSA-167290 Reactome: R-HSA-168325 Reactome: R-HSA-203927 Reactome: R-HSA-5578749 Reactome: R-HSA-5601884 Reactome: R-HSA-5617472 Reactome: R-HSA-674695 Reactome: R-HSA-6781823 Reactome: R-HSA-6781827 Reactome: R-HSA-6782135 Reactome: R-HSA-6782210 Reactome: R-HSA-6796648 Reactome: R-HSA-6803529 Reactome: R-HSA-6807505 Reactome: R-HSA-72086 Reactome: R-HSA-72163 Reactome: R-HSA-72165 Reactome: R-HSA-72203 Reactome: R-HSA-73776 Reactome: R-HSA-73779 Reactome: R-HSA-75953 Reactome: R-HSA-75955 Reactome: R-HSA-76042 Reactome: R-HSA-77075 Reactome: R-HSA-8851708 Reactome: R-HSA-9018519 Reactome: R-HSA-9670095
Cellular Localisation	Nucleus
Alternative Antibody Names	Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb11-A antibody Anti-Rna Polymerase Ii Subunit B11-A antibody Anti-Rpb11a antibody Anti-Dna-Directed Rna Polymerase Ii Subunit J-1 antibody Anti-Rna Polymerase Ii 13.3 Kda Subunit antibody Anti-POLR2J antibody Anti-POLR2J1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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