| Host: | Rabbit |
| Applications: | WB/IF |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-POLR2E (1-210) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLR2E |
| Gene ID: | 5434 |
| Uniprot ID: | RPAB1_HUMAN |
| Immunogen Region: | 1-210 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human POLR2E (NP_002686.2). |
| Immunogen Sequence: | MDDEEETYRLWKIRKTIMQL CHDRGYLVTQDELDQTLEEF KAQFGDKPSEGRPRRTDLTV LVAHNDDPTDQMFVFFPEEP KVGIKTIKVYCQRMQEENIT RALIVVQQGMTPSAKQSLVD MAPKYILEQFLQQELLINIT EHELVPEHVVMTKEEVTELL ARYKLRENQLPRIQAGDPVA RYFGIKRGQVVKIIRPSETA GRYITYRLVQ |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. |
| Protein Name | Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc1Rna Polymerases I - Ii - And Iii Subunit Abc1Dna-Directed Rna Polymerase Ii 23 Kda PolypeptideDna-Directed Rna Polymerase Ii Subunit ERpb5 HomologXap4 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-168325Reactome: R-HSA-1834949Reactome: R-HSA-203927Reactome: R-HSA-427413Reactome: R-HSA-5250924Reactome: R-HSA-5578749Reactome: R-HSA-5601884Reactome: R-HSA-5617472Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6803529Reactome: R-HSA-6807505Reactome: R-HSA-72086Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72203Reactome: R-HSA-73762Reactome: R-HSA-73772Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-73780Reactome: R-HSA-73863Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071Reactome: R-HSA-77075Reactome: R-HSA-8851708Reactome: R-HSA-9018519Reactome: R-HSA-9670095 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerases I - Ii - And Iii Subunit Rpabc1 antibodyAnti-Rna Polymerases I - Ii - And Iii Subunit Abc1 antibodyAnti-Dna-Directed Rna Polymerase Ii 23 Kda Polypeptide antibodyAnti-Dna-Directed Rna Polymerase Ii Subunit E antibodyAnti-Rpb5 Homolog antibodyAnti-Xap4 antibodyAnti-POLR2E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
