| Host: | Rabbit |
| Applications: | WB/IF/IP |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-POLR2D (1-142) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IF/ICC 1:10-1:100IP 1:50-1:200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLR2D |
| Gene ID: | 5433 |
| Uniprot ID: | RPB4_HUMAN |
| Immunogen Region: | 1-142 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1). |
| Immunogen Sequence: | MAAGGSDPRAGDVEEDASQL IFPKEFETAETLLNSEVHML LEHRKQQNESAEDEQELSEV FMKTLNYTARFSRFKNRETI ASVRSLLLQKKLHKFELACL ANLCPETAEESKALIPSLEG RFEDEELQQILDDIQTKRSF QY |
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RPB4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. |
| Protein Name | Dna-Directed Rna Polymerase Ii Subunit Rpb4Rna Polymerase Ii Subunit B4Dna-Directed Rna Polymerase Ii Subunit DRna Polymerase Ii 16 Kda SubunitRpb16 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-113418Reactome: R-HSA-167152Reactome: R-HSA-167158Reactome: R-HSA-167160Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167242Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-168325Reactome: R-HSA-203927Reactome: R-HSA-5578749Reactome: R-HSA-5601884Reactome: R-HSA-5617472Reactome: R-HSA-674695Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6796648Reactome: R-HSA-6803529Reactome: R-HSA-6807505Reactome: R-HSA-72086Reactome: R-HSA-72163Reactome: R-HSA-72165Reactome: R-HSA-72203Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-75955Reactome: R-HSA-76042Reactome: R-HSA-77075Reactome: R-HSA-8851708Reactome: R-HSA-9018519Reactome: R-HSA-9670095 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Ii Subunit Rpb4 antibodyAnti-Rna Polymerase Ii Subunit B4 antibodyAnti-Dna-Directed Rna Polymerase Ii Subunit D antibodyAnti-Rna Polymerase Ii 16 Kda Subunit antibodyAnti-Rpb16 antibodyAnti-POLR2D antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
