• Western blot analysis of lysates from HeLa cells, using POLI Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from HeLa cells using POLI antibody.
  • Western blot analysis of HeLa cells using DNA pol Iota Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POLI antibody (641-690 aa) (STJ92742)

SKU:
STJ92742

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA polymerase iota (641-690 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLI
Gene ID: 11201
Uniprot ID: POLI_HUMAN
Immunogen Region: 641-690 aa
Specificity: DNA pol Iota Polyclonal Antibody detects endogenous levels of DNA pol Iota protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human POLI at the amino acid range 641-690
Post Translational Modifications Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2.
Function Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Protein Name Dna Polymerase Iota
Eta2
Rad30 Homolog B
Database Links Reactome: R-HSA-5656121
Reactome: R-HSA-5656169
Cellular Localisation Nucleus
Binding To Ubiquitin Mediates Localization To Replication Forks After Uv-Induced Dna Damage
Alternative Antibody Names Anti-Dna Polymerase Iota antibody
Anti-Eta2 antibody
Anti-Rad30 Homolog B antibody
Anti-POLI antibody
Anti-RAD30B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance