Anti-POLH antibody (322-520) (STJ25041)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DNA polymerase eta (322-520) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLH |
| Gene ID | 5429 |
| Uniprot ID | POLH_HUMAN |
| Immunogen Region | 322-520 |
| Immunogen Sequence | SKNFPGKTALATREQVQWWL LQLAQELEERLTKDRNDNDR VATQLVVSIRVQGDKRLSSL RRCCALTRYDAHKMSHDAFT VIKNCNTSGIQTEWSPPLTM LFLCATKFSASAPSSSTDIT SFLSSDPSSLPKVPVTSSEA KTQGSGPAVTATKKATTSLE SFFQKAAERQKVKEASLSSL TAPTQAPMSNSPSKPSLPF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 322-520 of human DNA polymerase eta (NP_006493.1). |
Additional Info
| Post Translational Modifications | Monoubiquitinated by RCHY1/PIRH2. Ubiquitination depends on integrity of the UBZ3-type zinc finger domain and is enhanced by TRAIP. Ubiquitination inhibits the ability of PolH to interact with PCNA and to bypass UV-induced lesions. |
| Function | DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine. Particularly important for the repair of UV-induced pyrimidine dimers. Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci. |
| Protein Name | Dna Polymerase EtaRad30 Homolog AXeroderma Pigmentosum Variant Type Protein |
| Database Links | Reactome: R-HSA-110320Reactome: R-HSA-5656169Reactome: R-HSA-5685942 |
| Cellular Localisation | NucleusBinding To Ubiquitinated Pcna Mediates Colocalization To Replication Foci During Dna Replication And Persists At Sites Of Stalled Replication Forks Following Uv IrradiationAfter Uv IrradiationRecruited To Dna Damage Sites Within 1 HourTo A Maximum Of About 80%This Recruitment May Not Be Not Restricted To Cells Active In Dna ReplicationColocalizes With Traip To Nuclear Foci |
| Alternative Antibody Names | Anti-Dna Polymerase Eta antibodyAnti-Rad30 Homolog A antibodyAnti-Xeroderma Pigmentosum Variant Type Protein antibodyAnti-POLH antibodyAnti-RAD30 antibodyAnti-RAD30A antibodyAnti-XPV antibody |
Information sourced from Uniprot.org