• Immunofluorescence analysis of MCF7 cells using POLG2 antibody (STJ28778). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using POLG2 antibody (STJ28778) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using POLG2 antibody (STJ28778) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLG2 antibody (216-485) (STJ28778)

SKU:
STJ28778

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:10-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLG2
Gene ID: 11232
Uniprot ID: DPOG2_HUMAN
Immunogen Region: 216-485
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 216-485 of human POLG2 (NP_009146.2).
Immunogen Sequence: HPVFDTKQIRNGVKSIGEKT EASLVWFTPPRTSNQWLDFW LRHRLQWWRKFAMSPSNFSS SDCQDEEGRKGNKLYYNFPW GKELIETLWNLGDHELLHMY PGNVSKLHGRDGRKNVVPCV LSVNGDLDRGMLAYLYDSFQ LTENSFTRKKNLHRKVLKLH PCLAPIKVALDVGRGPTLEL RQVCQGLFNELLENGISVWP GYLETMQSSLEQLYSKYDEM SILFTVLVTETTLENGLIH
Function Accessory subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Acts as an allosteric regulator of the holoenzyme activities. Enhances the polymerase activity and the processivity of POLG by increasing its interactions with the DNA template. Suppresses POLG exonucleolytic proofreading especially toward homopolymeric templates bearing mismatched termini. Binds to single-stranded DNA.
Protein Name Dna Polymerase Subunit Gamma-2
Dna Polymerase Gamma Accessory 55 Kda Subunit
P55
Mitochondrial Dna Polymerase Accessory Subunit
Mtpolb
Polg-Beta
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Dna Polymerase Subunit Gamma-2 antibody
Anti-Dna Polymerase Gamma Accessory 55 Kda Subunit antibody
Anti-P55 antibody
Anti-Mitochondrial Dna Polymerase Accessory Subunit antibody
Anti-Mtpolb antibody
Anti-Polg-Beta antibody
Anti-POLG2 antibody
Anti-MTPOLB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance