| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POLG |
| Gene ID: |
5428 |
| Uniprot ID: |
DPOG1_HUMAN |
| Immunogen Region: |
540-720 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 540-720 of human DNA Polymerase gamma (NP_002684.1). |
| Immunogen Sequence: |
QQDVMARACLQKLKGTTELL PKRPQHLPGHPGWYRKLCPR LDDPAWTPGPSLLSLQMRVT PKLMALTWDGFPLHYSERHG WGYLVPGRRDNLAKLPTGTT LESAGVVCPYRAIESLYRKH CLEQGKQQLMPQEAGLAEEF LLTDNSAIWQTVEELDYLEV EAEAKMENLRAAVPGQPLAL T |
| Function | Catalytic subunit of DNA polymerase gamma solely responsible for replication of mitochondrial DNA (mtDNA). Replicates both heavy and light strands of the circular mtDNA genome using a single-stranded DNA template, RNA primers and the four deoxyribonucleoside triphosphates as substrates. Has 5' -> 3' polymerase activity. Functionally interacts with TWNK and SSBP1 at the replication fork to form a highly processive replisome, where TWNK unwinds the double-stranded DNA template prior to replication and SSBP1 covers the parental heavy strand to enable continuous replication of the entire mitochondrial genome. A single nucleotide incorporation cycle includes binding of the incoming nucleotide at the insertion site, a phosphodiester bond formation reaction that extends the 3'-end of the primer DNA, and translocation of the primer terminus to the post-insertion site. After completing replication of a mtDNA strand, mediates 3' -> 5' exonucleolytic degradation at the nick to enable proper ligation. Highly accurate due to high nucleotide selectivity and 3' -> 5' exonucleolytic proofreading. Proficiently corrects base substitutions, single-base additions and deletions in non-repetitive sequences and short repeats, but displays lower proofreading activity when replicating longer homopolymeric stretches. Exerts exonuclease activity toward single-stranded DNA and double-stranded DNA containing 3'-terminal mispairs. When a misincorporation occurs, transitions from replication to a pro-nucleolytic editing mode and removes the missincorporated nucleoside in the exonuclease active site. Proceeds via an SN2 nucleolytic mechanism in which Asp-198 catalyzes phosphodiester bond hydrolysis and Glu-200 stabilizes the leaving group. As a result the primer strand becomes one nucleotide shorter and is positioned in the post-insertion site, ready to resume DNA synthesis. Exerts 5'-deoxyribose phosphate (dRP) lyase activity and mediates repair-associated mtDNA synthesis (gap filling) in base-excision repair pathway. Catalyzes the release of the 5'-terminal 2-deoxyribose-5-phosphate sugar moiety from incised apurinic/apyrimidinic (AP) sites to produce a substrate for DNA ligase. The dRP lyase reaction does not require divalent metal ions and likely proceeds via a Schiff base intermediate in a beta-elimination reaction mechanism. |
| Protein Name | Dna Polymerase Subunit Gamma-13'-5' Exodeoxyribonuclease5'-Deoxyribose-Phosphate LyaseMitochondrial Dna Polymerase Catalytic SubunitPolg-Alpha |
| Cellular Localisation | MitochondrionMitochondrion MatrixMitochondrion Nucleoid |
| Alternative Antibody Names | Anti-Dna Polymerase Subunit Gamma-1 antibodyAnti-3'-5' Exodeoxyribonuclease antibodyAnti-5'-Deoxyribose-Phosphate Lyase antibodyAnti-Mitochondrial Dna Polymerase Catalytic Subunit antibodyAnti-Polg-Alpha antibodyAnti-POLG antibodyAnti-MDP1 antibodyAnti-POLG1 antibodyAnti-POLGA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
