| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA polymerase epsilon subunit 3 is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | POLE3 |
| Gene ID: | 54107 |
| Uniprot ID: | DPOE3_HUMAN |
| Specificity: | This antibody detects endogenous levels of POLE3 at Human, Mouse, Rat |
| Immunogen: | Synthesized peptide derived from human POLE3 |
| Function | Accessory component of the DNA polymerase epsilon complex. Participates in DNA repair and in chromosomal DNA replication. Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1. Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex. |
| Protein Name | Dna Polymerase Epsilon Subunit 3Arsenic-Transactivated ProteinAstpChromatin Accessibility Complex 17 Kda ProteinChrac-17Huchrac17Dna Polymerase Ii Subunit 3Dna Polymerase Epsilon Subunit P17 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-5651801Reactome: R-HSA-5656169Reactome: R-HSA-5685942Reactome: R-HSA-5696397Reactome: R-HSA-5696400Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-68952Reactome: R-HSA-68962 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Polymerase Epsilon Subunit 3 antibodyAnti-Arsenic-Transactivated Protein antibodyAnti-Astp antibodyAnti-Chromatin Accessibility Complex 17 Kda Protein antibodyAnti-Chrac-17 antibodyAnti-Huchrac17 antibodyAnti-Dna Polymerase Ii Subunit 3 antibodyAnti-Dna Polymerase Epsilon Subunit P17 antibodyAnti-POLE3 antibodyAnti-CHRAC17 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/101832/390042/STJ28552_1__58226.1713159925.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
