• Western blot analysis of lysates from Jurkat, HeLa, and HepG2 cells, using POFUT1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using POFUT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-POFUT1 antibody (331-380) (STJ94602)

SKU:
STJ94602

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GDP-fucose protein O-fucosyltransferase 1 (331-380) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POFUT1
Gene ID: 23509
Uniprot ID: OFUT1_HUMAN
Immunogen Region: 331-380
Specificity: O-FucT-1 Polyclonal Antibody detects endogenous levels of O-FucT-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human POFUT1. AA range:331-380
Tissue Specificity Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications N-glycosylated.
Function Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-S/T-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Protein Name Gdp-Fucose Protein O-Fucosyltransferase 1
Peptide-O-Fucosyltransferase 1
O-Fuct-1
Database Links Reactome: R-HSA-1912399
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Gdp-Fucose Protein O-Fucosyltransferase 1 antibody
Anti-Peptide-O-Fucosyltransferase 1 antibody
Anti-O-Fuct-1 antibody
Anti-POFUT1 antibody
Anti-FUT12 antibody
Anti-KIAA0180 antibody

Information sourced from Uniprot.org

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