• Human lymph node stained with anti-podoplannin antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of lymphatic endothelial cells..

Anti-Podoplanin antibody (24-126aa) [ZM31] (STJ180283)

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STJ180283

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Host: Mouse
Applications: IHC-P
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Podoplanin (24-126aa) is suitable for use in Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: ZM31
Conjugation: Unconjugated
Isotype: IgG1/Kappa
Formulation: Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin
Purification: Affinity purified
Dilution Range: 1:100‐200
Storage Instruction: Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C.
Gene Symbol: PDPN
Gene ID: 10630
Uniprot ID: PDPN_HUMAN
Immunogen Region: 24-126aa
Specificity: Positive control: Mesothelioma
Immunogen: Recombinant human Podoplanin (PDPN) protein fragment (around aa 24-126)
Tissue Specificity Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas.
Post Translational Modifications Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity. Disialylated at Thr-52.sialic acid is critical for platelet-aggregating activity and for CLEC1B interaction. The N-terminus is blocked. Cleaved by a metalloprotease within its extracellular (EC) domain, generating a membrane-bound C-terminal fragment (PCTF33) and an extracellular fragment. The resulting membrane-bound C-terminal fragment (PCTF33) is further processed between Val-150 and Val-151 by PSEN1/gamma-secretase generating the intracellular domain of podoplanin (PICD).
Function Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation induced by PDPN. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells. In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR.MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR.MSN and RDX) and MYL9 activation. Through binding with LGALS8 may participate in connection of the lymphatic endothelium to the surrounding extracellular matrix. In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1. Required for normal lung cell proliferation and alveolus formation at birth. Does not function as a water channel or as a regulator of aquaporin-type water channels. Does not have any effect on folic acid or amino acid transport.
Protein Name Podoplanin
Aggrus
Glycoprotein 36
Gp36
Pa2.26 Antigen
T1-Alpha
T1a Cleaved Into - 29kda Cytosolic Podoplanin Intracellular Domain
Picd
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-9827857
Cellular Localisation Podoplanin: Membrane
Single-Pass Type I Membrane Protein
Cell Projection
Lamellipodium Membrane
Filopodium Membrane
Microvillus Membrane
Ruffle Membrane
Membrane Raft
Apical Cell Membrane
Basolateral Cell Membrane
Invadopodium
Localized To Actin-Rich Microvilli And Plasma Membrane Projections Such As Filopodia
Lamellipodia And Ruffles
Association To The Lipid Rafts Is Required For Pdpn-Induced Epithelial To Mesenchymal Transition (Emt)
Colocalizes With Cd9 In Tetraspanin Microdomains
Localized At Invadopodium Adhesion Rings In Tumor Cell
Association To The Lipid Rafts Is Essential For Pdpn Recruitment To Invadopodia And Ecm Degradation
29kda Cytosolic Podoplanin Intracellular Domain: Cytoplasm
Cytosol
Alternative Antibody Names Anti-Podoplanin antibody
Anti-Aggrus antibody
Anti-Glycoprotein 36 antibody
Anti-Gp36 antibody
Anti-Pa2.26 Antigen antibody
Anti-T1-Alpha antibody
Anti-T1a Cleaved Into - 29kda Cytosolic Podoplanin Intracellular Domain antibody
Anti-Picd antibody
Anti-PDPN antibody
Anti-GP36 antibody
Anti-PSEC0003 antibody
Anti-PSEC0025 antibody

Information sourced from Uniprot.org

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