| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PODN |
| Gene ID: | 127435 |
| Uniprot ID: | PODN_HUMAN |
| Immunogen Region: | 67-210 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 67-210 of human PODN (NP_714914.2). |
| Immunogen Sequence: | LGPVLAVRAPGFGRSGGHSL SPEENEFAEEEPVLVLSPEE PGPGPAAVSCPRDCACSQEG VVDCGGIDLREFPGDLPEHT NHLSLQNNQLEKIYPEELSR LHRLETLNLQNNRLTSRGLP EKAFEHLTNLNYLYLANNKL TLAP |
| Tissue Specificity | Kidney, heart, liver, pancreas and vascular smooth muscle cells. Also detected in aortic intima (at protein level). |
| Post Translational Modifications | N-glycosylated. |
| Function | Negatively regulates cell proliferation and cell migration. |
| Protein Name | Podocan |
| Cellular Localisation | Isoform 1: SecretedExtracellular SpaceExtracellular MatrixIsoform 2: CytoplasmIsoform 3: CytoplasmIsoform 4: Cytoplasm |
| Alternative Antibody Names | Anti-Podocan antibodyAnti-PODN antibodyAnti-SLRR5A antibodyAnti-UNQ293 antibodyAnti-PRO332 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/98770/382819/STJ119985_1__92297.1713151316.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
