• Western blot analysis of extracts of various cell lines, using PODN antibody (STJ116521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PODN antibody (67-210) (STJ116521)

SKU:
STJ116521

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PODN (67-210) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PODN
Gene ID: 127435
Uniprot ID: PODN_HUMAN
Immunogen Region: 67-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 67-210 of human PODN (NP_714914.2).
Immunogen Sequence: LGPVLAVRAPGFGRSGGHSL SPEENEFAEEEPVLVLSPEE PGPGPAAVSCPRDCACSQEG VVDCGGIDLREFPGDLPEHT NHLSLQNNQLEKIYPEELSR LHRLETLNLQNNRLTSRGLP EKAFEHLTNLNYLYLANNKL TLAP
Tissue Specificity Kidney, heart, liver, pancreas and vascular smooth muscle cells. Also detected in aortic intima (at protein level).
Post Translational Modifications N-glycosylated.
Function Negatively regulates cell proliferation and cell migration.
Protein Name Podocan
Cellular Localisation Isoform 1: Secreted
Extracellular Space
Extracellular Matrix
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative Antibody Names Anti-Podocan antibody
Anti-PODN antibody
Anti-SLRR5A antibody
Anti-UNQ293 antibody
Anti-PRO332 antibody

Information sourced from Uniprot.org

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