• Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PNP antibody (1-289) (STJ11100761)

SKU:
STJ11100761

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PNP (1-289) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PNP
Gene ID: 4860
Uniprot ID: PNPH_HUMAN
Immunogen Region: 1-289
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2).
Immunogen Sequence: MENGYTYEDYKNTAEWLLSH TKHRPQVAIICGSGLGGLTD KLTQAQIFDYGEIPNFPRST VPGHAGRLVFGFLNGRACVM MQGRFHMYEGYPLWKVTFPV RVFHLLGVDTLVVTNAAGGL NPKFEVGDIMLIRDHINLPG FSGQNPLRGPNDERFGDRFP AMSDAYDRTMRQRALSTWKQ MGEQRELQEGTYVMVAGPSF ETVAECRVLQKLGADAVGMS TVPEVIVARHCGLRVFGFS
Tissue Specificity Expressed in red blood cells.overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology.
Function Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine.
Protein Name Purine Nucleoside Phosphorylase
Pnp
Inosine Phosphorylase
Inosine-Guanosine Phosphorylase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-74217
Reactome: R-HSA-74259
Reactome: R-HSA-9735763
Reactome: R-HSA-9755088
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Purine Nucleoside Phosphorylase antibody
Anti-Pnp antibody
Anti-Inosine Phosphorylase antibody
Anti-Inosine-Guanosine Phosphorylase antibody
Anti-PNP antibody
Anti-NP antibody

Information sourced from Uniprot.org

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