![Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89698/358183/STJ11100761_1__08836.1713122495.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89698/358183/STJ11100761_1__08836.1713122495.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PNP knockout (KO) 293T cells, using [KO Validated] PNP Rabbit polyclonal antibody (STJ11100761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PNP |
| Gene ID: | 4860 |
| Uniprot ID: | PNPH_HUMAN |
| Immunogen Region: | 1-289 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human PNP (NP_000261.2). |
| Immunogen Sequence: | MENGYTYEDYKNTAEWLLSH TKHRPQVAIICGSGLGGLTD KLTQAQIFDYGEIPNFPRST VPGHAGRLVFGFLNGRACVM MQGRFHMYEGYPLWKVTFPV RVFHLLGVDTLVVTNAAGGL NPKFEVGDIMLIRDHINLPG FSGQNPLRGPNDERFGDRFP AMSDAYDRTMRQRALSTWKQ MGEQRELQEGTYVMVAGPSF ETVAECRVLQKLGADAVGMS TVPEVIVARHCGLRVFGFS |
| Tissue Specificity | Expressed in red blood cells.overexpressed in red blood cells (cytoplasm) of patients with hereditary non-spherocytic hemolytic anemia of unknown etiology. |
| Function | Catalyzes the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Preferentially acts on 6-oxopurine nucleosides including inosine and guanosine. |
| Protein Name | Purine Nucleoside PhosphorylasePnpInosine PhosphorylaseInosine-Guanosine Phosphorylase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-74217Reactome: R-HSA-74259Reactome: R-HSA-9735763Reactome: R-HSA-9755088 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Purine Nucleoside Phosphorylase antibodyAnti-Pnp antibodyAnti-Inosine Phosphorylase antibodyAnti-Inosine-Guanosine Phosphorylase antibodyAnti-PNP antibodyAnti-NP antibody |
Information sourced from Uniprot.org