• Western blot analysis of various lysates, using PLP1 rabbit polyclonal antibody (STJ116464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Perform microwave antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IF staining protocol. Immunofluorescence analysis of rat brain using PLP1 rabbit polyclonal antibody (STJ116464) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PLP1 antibody (102-148) (STJ116464)

SKU:
STJ116464

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLP1 (102-148) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLP1
Gene ID: 5354
Uniprot ID: MYPR_HUMAN
Immunogen Region: 102-148
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 102-148 of human PLP1 (NP_955772.1).
Immunogen Sequence: GDYKTTICGKGLSATFVGIT YALTVVWLLVFACSAVPVYI YFNTWTT
Function This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.
Protein Name Myelin Proteolipid Protein
Plp
Lipophilin
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Myelin Membrane
Colocalizes With Sirt2 In Internodal Regions
At Paranodal Axoglial Junction And Schmidt-Lanterman Incisures Of Myelin Sheat
Alternative Antibody Names Anti-Myelin Proteolipid Protein antibody
Anti-Plp antibody
Anti-Lipophilin antibody
Anti-PLP1 antibody
Anti-PLP antibody

Information sourced from Uniprot.org

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