• Immunofluorescence analysis of C6 cells using Perilipin-2 Rabbit polyclonal antibody (STJ28198) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from HepG2 cells, using Perilipin-2 Rabbit polyclonal antibody (STJ28198) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from Rat heart, using Perilipin-2 Rabbit polyclonal antibody (STJ28198) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PLIN2 antibody (168-437) (STJ28198)

SKU:
STJ28198

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:100-1:500
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLIN2
Gene ID: 123
Uniprot ID: PLIN2_HUMAN
Immunogen Region: 168-437
Specificity: A synthetic peptide corresponding to a sequence within amino acids 168-437 of human Perilipin-2 (NP_001113.2).
Immunogen Sequence: MMQLVSSGVENALTKSELLV EQYLPLTEEELEKEAKKVEG FDLVQKPSYYVRLGSLSTKL HSRAYQQALSRVKEAKQKSQ QTISQLHSTVHLIEFARKNV YSANQKIQDAQDKLYLSWVE WKRSIGYDDTDESHCAEHIE SRTLAIARNLTQQLQTTCHT LLSNIQGVPQNIQDQAKHMG VMAGDIYSVFRNAASFKEVS DSLLTSSKGQLQKMKESLDD VMDYLVNNTPLNWLVGPFY
Tissue Specificity Milk lipid globules.
Post Translational Modifications Acylated.primarily with C14, C16 and C18 fatty acids. Phosphorylation at Tyr-232 by isoform 1 of CHKA (CHKalpha2) promotes dissociation from lipid droplets: dissociation is followed by recruitment of autophagosome machinery to lipid droplets and subsequent lipid droplet lipolysis.
Function Structural component of lipid droplets, which is required for the formation and maintenance of lipid storage droplets.
Protein Name Perilipin-2
Adipophilin
Adipose Differentiation-Related Protein
Adrp
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-9613354
Reactome: R-HSA-9613829
Reactome: R-HSA-9615710
Cellular Localisation Membrane
Peripheral Membrane Protein
Lipid Droplet
Alternative Antibody Names Anti-Perilipin-2 antibody
Anti-Adipophilin antibody
Anti-Adipose Differentiation-Related Protein antibody
Anti-Adrp antibody
Anti-PLIN2 antibody
Anti-ADFP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance