Confocal imaging of paraffin-embedded Rat brown fat tissue using Perilipin A Rabbit monoclonal antibody (STJ11102149, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PLIN1 antibody (400-500) [S9MR] (STJ11102149)

SKU:
STJ11102149
£146.50 - £642.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:100-1:1000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLIN1
Gene ID: 5346
Uniprot ID: PLIN1_HUMAN
Immunogen Region: 400-500
Specificity: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human Perilipin A (O60240).
Immunogen Sequence: HYVPLPRLSLMEPESEFRDI DNPPAEVERREAERRASGAP SAGPEPAPRLAQPRRSLRSA QSPGAPPGPGLEDEVATPAA PRPGFPAVPREKPKRRVSDS F
Tissue Specificity Detected in adipocytes from white adipose tissue (at protein level). Detected in visceral adipose tissue and mammary gland.
Post Translational Modifications Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
Function Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.
Protein Name Perilipin-1
Lipid Droplet-Associated Protein
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-381340
Reactome: R-HSA-9031528
Cellular Localisation Endoplasmic Reticulum
Lipid Droplet
Lipid Droplet Surface-Associated
Alternative Antibody Names Anti-Perilipin-1 antibody
Anti-Lipid Droplet-Associated Protein antibody
Anti-PLIN1 antibody
Anti-PERI antibody
Anti-PLIN antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ118726 Anti-PLIN1 antibody (400-500) (STJ118726) Rabbit Polyclonal Human WB/ELISA
STJ11101299 Anti-G6PD antibody (400-500) [S9MR] (STJ11101299) Rabbit Monoclonal Human WB/ELISA
STJ11101359 Anti-GABRB2 antibody (300-400) [S9MR] (STJ11101359) Rabbit Monoclonal Human/Mouse/Rat WB/ELISA
STJ11101369 Anti-CBS antibody (400-551) [S9MR] (STJ11101369) Rabbit Monoclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA
STJA0009775 Anti-PLIN1 antibody (STJA0009775) Rabbit Polyclonal Human/Mouse/Rat WB
STJ11103805 Anti-LMNA antibody (400-500) (STJ11103805) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA/ChIP
STJ11105704 Anti-SYN1 antibody (400-500) (STJ11105704) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
!