| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-5 3 exonuclease PLD3 (326-375 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:5000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLD3 |
| Gene ID: |
23646 |
| Uniprot ID: |
PLD3_HUMAN |
| Immunogen Region: |
326-375 aa |
| Specificity: |
PC-PLD3 Polyclonal Antibody detects endogenous levels of PC-PLD3 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human PLD3 at the amino acid range 326-375 |
| Post Translational Modifications | N-glycosylated. Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex. Ubiquitinated at N-terminus. Ubiquitination mediates sorting into lysosomes. |
| Function | 5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4. May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting. |
| Protein Name | 5'-3' Exonuclease Pld3Choline Phosphatase 3Hindiii K4l HomologHu-K4Phosphatidylcholine-Hydrolyzing Phospholipase D3Phospholipase D3Pld 3 |
| Database Links | Reactome: R-HSA-1483148Reactome: R-HSA-2029485 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane ProteinLysosome LumenEarly Endosome MembraneLate Endosome MembraneGolgi Apparatus MembraneEndosome MembraneLocalizes To Er-Associated Vesicles In Differentiating MyotubesThe Soluble Form In Lysosome Arises By Proteolytic Processing Of The Membrane-Bound FormColocalizes With App In Endosomes |
| Alternative Antibody Names | Anti-5'-3' Exonuclease Pld3 antibodyAnti-Choline Phosphatase 3 antibodyAnti-Hindiii K4l Homolog antibodyAnti-Hu-K4 antibodyAnti-Phosphatidylcholine-Hydrolyzing Phospholipase D3 antibodyAnti-Phospholipase D3 antibodyAnti-Pld 3 antibodyAnti-PLD3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
