• Immunohistochemistry analysis of paraffin-embedded rat brain using PLC beta 3 (PLC beta 3 (PLCB3) ) Rabbit polyclonal antibody (STJ112274) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PLC beta 3 (PLC beta 3 (PLCB3) ) antibody (STJ112274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PLCB3 antibody (1010-1234) (STJ112274)

SKU:
STJ112274

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLCB3 (1010-1234) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLCB3
Gene ID: 5331
Uniprot ID: PLCB3_HUMAN
Immunogen Region: 1010-1234
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1010-1234 of human PLC beta 3 (PLCB3) (NP_001303243.1).
Immunogen Sequence: GALGGAADVEDTKEGEDEAK RYQEFQNRQVQSLLELREAQ VDAEAQRRLEHLRQALQRLR EVVLDANTTQFKRLKEMNER EKKELQKILDRKRHNSISEA KMRDKHKKEAELTEINRRHI TESVNSIRRLEEAQKQRHDR LVAGQQQVLQQLAEEEPKLL AQLAQECQEQRARLPQEIRR SLLGEMPEGLGDGPLVACAS NGHAPGSSGHLSGADSESQE ENTQL
Function The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.
Protein Name 1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-3
Phosphoinositide Phospholipase C-Beta-3
Phospholipase C-Beta-3
Plc-Beta-3
Database Links Reactome: R-HSA-112043
Reactome: R-HSA-1855204
Reactome: R-HSA-399997
Reactome: R-HSA-4086398
Reactome: R-HSA-416476
Reactome: R-HSA-418217
Reactome: R-HSA-434316
Reactome: R-HSA-500657
Cellular Localisation Cytoplasm
Membrane
Nucleus
And Particulate Fractions
Alternative Antibody Names Anti-1-Phosphatidylinositol 4 -5-Bisphosphate Phosphodiesterase Beta-3 antibody
Anti-Phosphoinositide Phospholipase C-Beta-3 antibody
Anti-Phospholipase C-Beta-3 antibody
Anti-Plc-Beta-3 antibody
Anti-PLCB3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance