• Western blot analysis of Arabidopsis thaliana, using Actin (Plant Specific) Rabbit monoclonal antibody (STJ11105267) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of lysates from Oryza sativa, using Actin (Plant Specific) Rabbit monoclonal antibody (STJ11105267) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Plant Actin antibody (1-100) [S5267RM] (STJ11105267)

SKU:
STJ11105267

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Host: Rabbit
Applications: WB
Reactivity: O.sativa/A.thaliana
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Plant Actin (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S5267RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of Actin (Plant Specific) (NP_190236.1).
Immunogen Sequence: MADGEDIQPLVCDNGTGMVK AGFAGDDAPRAVFPSIVGRP RHTGVMVGMGQKDAYVGDEA QSKRGILTLKYPIEHGIVNN WDDMEKIWHHTFYNELRVAP
Background Actin is an ancient conserved protein that is encoded by multiple isovariants in multi-cellular organisms. There are eight functional actin genes in the Arabidopsis genome.

Information sourced from Uniprot.org

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