Anti-PLA2G1B antibody (23-148) (STJ27431)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PLA2G1B (23-148) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLA2G1B |
| Gene ID | 5319 |
| Uniprot ID | PA21B_HUMAN |
| Immunogen Region | 23-148 |
| Immunogen Sequence | AVWQFRKMIKCVIPGSDPFL EYNNYGCYCGLGGSGTPVDE LDKCCQTHDNCYDQAKKLDS CKFLLDNPYTHTYSYSCSGS AITCSSKNKECEAFICNCDR NAAICFSKAPYNKAHKNLDT KKYCQS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 23-148 of human PLA2G1B (NP_000919.1). |
Additional Info
| Tissue Specificity | Selectively expressed in pancreas, lung, liver and kidney. Also detected at lower levels in ovary and testis. |
| Post Translational Modifications | Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically. |
| Function | Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Upon binding to the PLA2R1 receptor can regulate podocyte survival and glomerular homeostasis. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity. |
| Protein Name | Phospholipase A2Group Ib Phospholipase A2Phosphatidylcholine 2-Acylhydrolase 1b |
| Database Links | Reactome: R-HSA-1482788Reactome: R-HSA-1482801Reactome: R-HSA-1482839Reactome: R-HSA-1482922Reactome: R-HSA-1482925Reactome: R-HSA-1483166Reactome: R-HSA-9925561 |
| Cellular Localisation | SecretedSecreted From Pancreatic Acinar Cells In Its Inactive Form |
| Alternative Antibody Names | Anti-Phospholipase A2 antibodyAnti-Group Ib Phospholipase A2 antibodyAnti-Phosphatidylcholine 2-Acylhydrolase 1b antibodyAnti-PLA2G1B antibodyAnti-PLA2 antibodyAnti-PLA2A antibodyAnti-PPLA2 antibody |
Information sourced from Uniprot.org