Anti-PKP2 antibody (632-681 aa) (STJ95149)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PKP2 at the amino acid range 632-681
General Information
| Short Description | Rabbit polyclonal anti-Plakophilin-2 (632-681 aa) for use in WB, ELISA and IHC in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PKP2 |
| Gene ID | 5318 |
| Uniprot ID | PKP2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PKP2 at the amino acid range 632-681 |
| Immunogen Region | 632-681 aa |
| Specificity | Plakophilin 2 Polyclonal Antibody detects endogenous levels of Plakophilin 2 protein. |
Additional Info
| Function | A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins. Required to maintain gingival epithelial barrier function. Important component of the desmosome that is also required for localization of desmosome component proteins such as DSC2, DSG2 and JUP to the desmosome cell-cell junction. Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls. Loss of desmosome cell junctions leads to mis-localization of DSP and DSG2 resulting in disruption of cell-cell adhesion and disordered intermediate filaments. Modulates profibrotic gene expression in cardiomyocytes via regulation of DSP expression and subsequent activation of downstream TGFB1 and MAPK14/p38 MAPK signaling. Required for cardiac sodium current propagation and electrical synchrony in cardiac myocytes, via ANK3 stabilization and modulation of SCN5A/Nav1.5 localization to cell-cell junctions. Required for mitochondrial function, nuclear envelope integrity and positive regulation of SIRT3 transcription via maintaining DES localization at its nuclear envelope and cell tip anchoring points, and thereby preserving regulation of the transcriptional program. Maintenance of nuclear envelope integrity protects against DNA damage and transcriptional dysregulation of genes, especially those involved in the electron transport chain, thereby preserving mitochondrial function and protecting against superoxide radical anion generation. Binds single-stranded DNA (ssDNA). May regulate the localization of GJA1 to gap junctions in intercalated disks of the heart. Involved in the inhibition of viral infection by influenza A viruses (IAV). Acts as a host restriction factor for IAV viral propagation, potentially via disrupting the interaction of IAV polymerase complex proteins. |
| Protein Name | Plakophilin-2 |
| Database Links | Reactome: R-HSA-6805567Reactome: R-HSA-6809371 |
| Cellular Localisation | NucleusCell JunctionDesmosomeCytoplasmColocalizes With Ctnna3 And Scn5a/Nav15 At Intercalated Disks In The Heart |
| Alternative Antibody Names | Anti-Plakophilin-2 antibodyAnti-PKP2 antibody |
Information sourced from Uniprot.org