Anti-PKMYT1 antibody (75-362) (STJ11103359)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PKMYT1 (75-362) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PKMYT1 |
| Gene ID | 9088 |
| Uniprot ID | PMYT1_HUMAN |
| Immunogen Region | 75-362 |
| Immunogen Sequence | HQLQPRRVSFRGEASETLQS PGYDPSRPESFFQQSFQRLS RLGHGSYGEVFKVRSKEDGR LYAVKRSMSPFRGPKDRARK LAEVGSHEKVGQHPCCVRLE QAWEEGGILYLQTELCGPSL QQHCEAWGASLPEAQVWGYL RDTLLALAHLHSQGLVHLDV KPANIFLGPRGRCKLGDFGL LVELGTAGAGEVQEGDPRYM APELLQGSYGTAADVFSLGL TILEVACNMELPHGGEGWQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 75-362 of human PKMYT1 (NP_004194.3). |
Additional Info
| Post Translational Modifications | Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity. |
| Function | Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. |
| Protein Name | Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory KinaseMyt1 Kinase |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69273Reactome: R-HSA-69478 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinGolgi Apparatus Membrane |
| Alternative Antibody Names | Anti-Membrane-Associated Tyrosine- And Threonine-Specific Cdc2-Inhibitory Kinase antibodyAnti-Myt1 Kinase antibodyAnti-PKMYT1 antibodyAnti-MYT1 antibody |
Information sourced from Uniprot.org