| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Piwi-Like Protein 2 (100-200) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIWIL2 |
| Gene ID: |
55124 |
| Uniprot ID: |
PIWL2_HUMAN |
| Immunogen Region: |
100-200 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human PIWIL2 (Q8TC59). |
| Immunogen Sequence: |
LGRGLSANLVRKDREELSPT FWDPKVLAAGDSKMAETSVG WSRTLGRGSSDASLLPLGRA AGGISREVDKPPCTFSTPSR GPPQLSSPPALPQSPLHSPD R |
| Tissue Specificity | Expressed in adult testis and in most tumors. |
| Post Translational Modifications | Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. |
| Function | Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4. PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded. Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Represses circadian rhythms by promoting the stability and activity of core clock components BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins. |
| Protein Name | Piwi-Like Protein 2Cancer/Testis Antigen 80Ct80 |
| Database Links | Reactome: R-HSA-5601884 |
| Cellular Localisation | CytoplasmPresent In Chromatoid BodyProbable Component Of The Meiotic NuageAlso Named P GranuleA Germ-Cell-Specific Organelle Required To Repress Transposon Activity During Meiosis |
| Alternative Antibody Names | Anti-Piwi-Like Protein 2 antibodyAnti-Cancer/Testis Antigen 80 antibodyAnti-Ct80 antibodyAnti-PIWIL2 antibodyAnti-HILI antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
