| Host: |
Mouse |
| Applications: |
IHC-P |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-PIT-1/POU1F1 (Full length) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
ZM385 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG2b/Kappa |
| Formulation: |
Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Purification: |
Affinity purified |
| Dilution Range: |
1:100-200 |
| Storage Instruction: |
Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
| Immunogen Region: |
Full length |
| Specificity: |
Positive Control: Pituitary |
| Immunogen: |
Recombinant human full-length POU1F1 protein |
| Background | Transcriptional regulators play a critical role in development by mediating tissue-and cell-specific transcription. POU domain factors are transcriptional regulators characterized by a bipartite DNA binding domain consisting of two highly conserved regions, tethered by a variable linker of 14-26 amino acids. Pit-1, also known as growth hormone factor-1 (GHF-1) , a member of the POU homeodomain family, is essential for the normal development of the anterior pituitary gland, where it is required to form of somatotropes, lactotropes, and thyrotropes. In somatotropes and lactotropes, Pit-1 activates the production of growth hormone and prolactin, respectively. In addition, Pit-1 acts as a repressor of gene expression, which allows for the differentiation of specific cell types. Pit-1 is expressed as two alternatively spliced products, designated Pit-1a and Pit-1b, which differ in their trans-activation ability. Mutations in the Pit-1 gene are believed to result in combined pituitary hormone deficiency (CPHD) for growth hormone, Prolactin, and thyroid-stimulating hormone. The gene which encodes Pit-1 maps to human chromosome 3p11.2. |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
